Multiplexing-Capable Immunobiosensor Based on Competitive Immunoassay
نویسندگان
چکیده
منابع مشابه
Label Free QCM Immunobiosensor for AFB1 Detection Using Monoclonal IgA Antibody as Recognition Element
This study introduces the use of an IgA isotype aflatoxin (AF) specific monoclonal antibody for the development of a highly sensitive Quartz Crystal Microbalance (QCM) immunobiosensor for the detection of AF in inhibitory immunoassay format. The higher molecular weight of IgA antibodies proved an advantage over commonly used IgG antibodies in label free immunobiosensor measurements. IgA and IgG...
متن کاملAnalysis of oxidative stress biomarkers using a simultaneous competitive/non-competitive micromosaic immunoassay.
Immunoassays represent a core workhorse methodology for many applications ranging from clinical diagnostics to environmental monitoring. In traditional formats such as the enzyme linked immunosorbent assay (ELISA), analytes are measured singly or in small sets. As more biomarkers are identified for disease states, there is a need to develop methods that can measure multiple markers simultaneous...
متن کاملUltrasensitive photoelectrochemical immunoassay through tag induced exciton trapping.
The development of photoelectrochemical (PEC) sensors with novel principles is of significance in realizing sensitive and low-cost detection. This work uses CuO NPs labeled antibody to construct a simple and sensitive sandwich-type immunobiosensor for the detection of protein. The detection signal is produced by dissolving the CuO NPs to release copper ions, which are then added on a quantum do...
متن کاملMultiplexed immunoassays by flow cytometry for detection of clenbuterol, chloramphenicol and sulfadimidine with high sensitivity and selectivity
An accurate, rapid and cost-effective detection on the environmental hazardous chemicals is the cornerstone of efficient food safe management. Here we discuss the relevance of an emerging technology, multiplexed competitive immunoassays read by flow cytometry, for the detection of Clenbuterol, Chloramphenicol and sulfadimidine. In these assays, multiple fluorescent microspheres, conjugated to d...
متن کاملCompetitive idiotype--anti-idiotype immunoassay for adenosine deaminase binding protein in urine.
This competitive immunoassay, based on inhibition by antigen of the idiotype-anti-idiotype interaction, detects adenosine deaminase binding protein (ABP), and involves use of monoclonal anti-idiotype antibodies prepared to a monoclonal antibody specific for ABP. The conditions for this new type of competitive immunoassay are investigated. This competitive immunoassay is as sensitive and reprodu...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2017